ICAR Research Data Repository for Knowledge Management
Kanamycin sensitivity of cultured tissues of Piper nigrum L.
DSpice at Indian Institute of Spices Research
View Archive Info| Field | Value | |
| Creator |
SASIKUMAR, B
VELUTHAMBI, K |
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| Date |
2006-09-16T09:11:16Z
2006-09-16T09:11:16Z 1994-12 |
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| Identifier |
Journal of Spices & Aromatic Crops 3 (2) : 158-160, 1994
0971-3328 http://hdl.handle.net/123456789/61 |
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| Description |
The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 µg ml-1 and above concentrations of kanamycin suggesting that 50 µg ml-1 is the minimum concentration needed to select the transformed tissues.
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| Format |
109959 bytes
application/pdf |
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| Language |
en
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| Publisher |
Indian Society for Spices
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| Subject |
Agrobacterium
black pepper callus kanamycin sensitivity Piper nigrum |
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| Title |
Kanamycin sensitivity of cultured tissues of Piper nigrum L.
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| Type |
Article
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