Record Details

Embryogenesis and plant regeneration from ovary derived callus cultures of ginger (Zingiber officinale Rosc.)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator NIRMAL BABU, K
SAMSUDEEN, K
RATNAMBAL, M J
RAVINDRAN, P N
 
Date 2006-09-19T06:35:15Z
2006-09-19T06:35:15Z
1996-12
 
Identifier Journal of Spices and Aromatic Crops 5 (2) : 134-138, 1996
0971-3328
http://hdl.handle.net/123456789/74
 
Description Ovary explants from 1-2 week old flowers of ginger (Zingiber officinale) developed profuse callus in Murashige and Skoog medium supple¬mented either with 2,4-dichlorophenoxyacetic acid (lmgl-1) alone or with 2,4-dichlorophenoxyacetic acid (0.5 mgl-1) and benzyladenine (lmgl-1). The callus later turned embryogenic and produced white globular embryoid like structures when cultured on modified Murashige and Skoog medium supplemented with benzyladenine (10 mgl-' ) and 2,4-dichlorophenoxyacetic acid (0.2 mgl-1). The embroyoid formation was more pronounced when growth regulators were removed from the culture medium after initial embryogenesis. Some of the embryoids developed into complete plantlets. The primary embryoids directly produced secondary embryoids in subsequent cultures on growth regulator free medium. The individual embryoids developed into plantlets with better rooting when a-naphthalene acetic acid (1 mgl-l) was added to the culture medium. About 80 per cent of these plantlets were established in soil.
 
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application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Title Embryogenesis and plant regeneration from ovary derived callus cultures of ginger (Zingiber officinale Rosc.)
 
Type Article