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Isolation of protoplasts from cardamom (Elettaria cardamomum Maton.) and ginger (Zingiber officinale Rosc.)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator GEETHA, S P
NIRMAL BABU, K
REMA, J
RAVINDRAN, P N
PETER, K V
 
Date 2006-09-26T08:54:04Z
2006-09-26T08:54:04Z
2000
 
Identifier Journal of Spices and Aromatic Crops 9 (1) : 23-30 (2000)
0971-3328
http://hdl.handle.net/123456789/106
 
Description Protoplasts were isolated from leaf mesophyll tissue, collected from in vitro grown plantlets and cell suspension cultures of cardamom (Elettaria cardamomum) and ginger (Zingiber officinale). In cardamom, a protoplast yield of 3.5 x 105/g of leaf tissue was obtained when incubated in an enzyme solution containing 0.5% macerozyme R10, 2% cellulase Onozuka R10 and 9% mannitol for 18-20 h at 25°C in dark. The yield of protoplasts from cell suspension culture was 1.5 x 105 /g tissue, when incubated in 1% macerozyme RIO and 2% cellulase Onozuka R10 for 24 h at 25°C with gentle shaking at 53 rpm in dark. The viability of leaf mesophyll protoplast was 75% and that of cell suspension was 40% on Evan's blue staining. In ginger, a protoplast yield of 2.5 x 105 /g of leaf tissue was obtained on digestion in an enzyme solution containing 0.5% macerozyme R10, 3% hemicellulase and 5% cellulase Onozuka R10, when incubated for 10 h at 15°C followed by 6 h at 30°C. The protoplast viability was 55%. Protoplast yield from cell suspension culture was 1 x 105 /g of callus when digested with an enzyme solution of 1% macerozyme R10, 3% hemicellulase and 6% cellulase Onozuka R10 and incubated for lOh at 15°C and later at 30°C for 8 h. Seventy two per cent of the protoplasts were viable. The protoplasts from both the species could be cultured and made to develop up to microcalli stage.
 
Format 937167 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Subject cardamom
Elettaria cardamomum
ginger
protoplast
Zingiber officinale
 
Title Isolation of protoplasts from cardamom (Elettaria cardamomum Maton.) and ginger (Zingiber officinale Rosc.)
 
Type Article