Record Details

Detection of Ralstonia solanacearum in ginger rhizomes using post-enrichment NCM-ELISA

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator KUMAR, A
SARMA, Y R
PRIOU, S
 
Date 2006-09-27T10:31:06Z
2006-09-27T10:31:06Z
2002-06
 
Identifier Journal of Spices and Aromatic Crops 11 (1) : 35-40 (2002)
0971-3328
http://hdl.handle.net/123456789/120
 
Description Bacterial wilt, caused by Ralstonia solanacearum, is an important production constraint in ginger. The pathogen is seed rhizome borne and the low level of inoculum is very difficult to be detected and monitored by conventional methods. Pathogen detection and selection of healthy planting material is an important prerequisite for the production of a healthy crop of ginger in the field. In the present work we have evaluated the suitability of NCM-ELISA kit, developed at International Potato Center (CIP), Lima, Peru, for detecting bacterial wilt pathogen in ginger. The result indicated that the antibodies developed at CIP for potato strain of R. solanacearum was sensitive enough to detect R. solanacearum from ginger, chilli, Chromolaena and tomato. The sensitivity of the kit was determined to be 42 cells per ml of ginger extract when ELISA was performed after enrichment in selective medium. We have also tested the specificity of antibodies and found that the antibodies were specific for R. solanacearum. The extraction protocol (citrate buffer at pH 5.6) developed for potato was found to be suitable for ginger also.
 
Format 472806 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Subject bacterial wilt
ginger
NCM-ELISA
Ralstonia solanacearum
rhizome borne disease
 
Title Detection of Ralstonia solanacearum in ginger rhizomes using post-enrichment NCM-ELISA
 
Type Article