ICAR Research Data Repository for Knowledge Management
A method for isolation and reverse transcription of high quality RNA from Piper species
DSpice at Indian Institute of Spices Research
View Archive Info| Field | Value | |
| Creator |
JOHNSON, GEORGE K
SANDEEP VARMA, R GANGA, G ANANDARAJ, M PARTHASARATHY, V A |
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| Date |
2006-09-28T11:43:45Z
2006-09-28T11:43:45Z 2005-06 |
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| Identifier |
Journal of Spices and Aromatic Crops Vol. 14 (1) : 10-14 (2005)
0971-3328 http://hdl.handle.net/123456789/133 |
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| Description |
A rapid and efficient protocol for isolation of RNA from Piper leaves is described. RNA was extracted from two Piper species namely, P. nigrum and P. colubrinum, using strong denaturing buffer containing guanidinum thiocyanate and polyvinylpyrollidone followed by precipitation with pre-cooled sodium acetate and extraction with phenol:chloroform:isoamylalcohol (25:24:1). RNA was precipitated with isopropanol. The extraction procedure reliably yielded high quality RNA suitable for reverse transcription experiments.
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| Format |
391984 bytes
application/pdf |
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| Language |
en
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| Publisher |
Indian Society for Spices
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| Subject |
Piper spp.
reverse transcription RNA isolation |
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| Title |
A method for isolation and reverse transcription of high quality RNA from Piper species
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| Type |
Article
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