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β-Cyfluthrin biodegradation gene(s) associated in plasmid of Pseudomonas stutzeri (strain S1)

Indian Agricultural Research Journals

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Title β-Cyfluthrin biodegradation gene(s) associated in plasmid of Pseudomonas stutzeri (strain S1)
 
Creator SINGH, JITENDRA
SINGH, DILEEP K
SANDHU, KIRAN VEER
JHA, SUNIL KUMAR
GOPAL, MADHUBAN
 
Subject β-cyfluthrin
Biodegradation
Plasmid DNA
Pseudomonas stutzeri
 
Description Pseudomonas stutzeri strain S1 was found capable for biodegradation of β-cyfluthrin. Results indicated that the b- cyfluthrin degrading gene(s) of P. stutzeri strain S1 were located on a plasmid. This plasmid already had the gene(s) for nalidixic acid resistance. Another gene, Tn5 was inserted into the plasmid from Escherichia coli S17. The mutated P. stutzeri acquired resistance for nalidixic acid and kanamycin antibiotics and became incapable to degrade β-cyfluthrin. Probably insertion of Tn5 distressed the sequence of β-cyfluthrin degrading gene. This mutant was assayed to locate the β-cyfluthrin biodegrading gene. The mutant was able to transfer the Tn5 inserted plasmid in another strain of E. coli DH5a, which was confirmed by acquiring kanamycin resistance in E. coli (DH5a) and PCR analysis. Plasmid of the P. stutzeri S1 was eliminated using ethidium bromide along with heat shock treatment, and then this strain lost biodegradation activity of β-cyfluthrin. Thus, it has been validated that the biodegradation gene(s) were present on the plasmid. The plasmid was identified as a DNA circular molecule of 18.0 kb size.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-10-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAgS/article/view/24161
10.56093/ijas.v82i10.24161
 
Source The Indian Journal of Agricultural Sciences; Vol. 82 No. 10 (2012); 831–4
2394-3319
0019-5022
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAgS/article/view/24161/11453
 
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