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Cloning of lichenase gene into Bacillus subtilis and partial characterization of the enzyme

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Field Value
 
Title Cloning of lichenase gene into Bacillus subtilis and partial characterization of the enzyme
 
Creator Mazi, Gamze
Baylan, Makbule
 
Subject Feed conversion rate
β-1,3(4)-Glucanase
Recombinant DNA
 
Description 56-61
Enzymes used as feed additives increase the ability of animals to benefit from feed. From these enzymes, animals fed with lichenase enzyme can produce animal foods with high immunity and performance. Therefore, it is possible to obtain more healthy, high quality products in a shorter time. In this study, the recombinant vector pNW33N carrying the lichenase gene of Streptococcus bovis genome was transferred into Bacillus subtilis RSKK245 strain via electroporation technique. Besides, the DNA band of lichenase obtained from recombinant vector pNW33N/Lichenase after restriction endonucleases was observed on agarose gel. Enzymatic activity sites around B. subtilis RSKK245 colonies are shown by staining with Congo-red. The molecular weight of the enzyme was determined as 26 kDa via SDS-PAGE and zymogram analysis. This study has successfully demonstrated expression of lichenase gene in B. subtilis RSKK245 strain.
 
Date 2023-12-26T11:17:38Z
2023-12-26T11:17:38Z
2023-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63086
https://doi.org/10.56042/ijeb.v62i01.6512
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(01) [Jan 2024]