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Effect of silver nanoparticles on antibody response against recombinant VP2 protein of Infectious bursal disease virus

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Title Effect of silver nanoparticles on antibody response against recombinant VP2 protein of Infectious bursal disease virus
 
Creator Awandkar, Sudhakar
Tembhurne, Prabhakar A.
Kurkure, Nitin V.
Chaudhari, Sandeep P.
Ingle, Vijay C.
 
Subject AgNP
VP2
Montanide oil
IgY
 
Description 7-16
A strong humoral response against Infectious bursal disease virus (IBDV) in breeder chickens transfers protective maternal
immunity in chicks, and protect them from the disease. The present study explains the modulatory effect of silver nanoparticles
on humoral immune response against recombinant VP2 (rVP2) protein of IBDV. The hypervariable VP2 gene segment of field
IBDV was amplified and cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated
rVP2 protein expressed in E. coli BL32DE3 cells, showed reactivity with specific anti VP2 chicken antibodies. The results of
immunoblot revealed its utility in serological diagnosis. The rVP2 protein was evaluated for immunogenic potential by
vaccinating the chickens with and without silver nanoparticles (AgNP). The titers induced by rVP2 protein blended with
montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was
highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and AgNP than in chickens vaccinated
with conventional vaccines or rVP2 protein in montanide oil without AgNP. The results represent Infectious bursal disease
virus rVP2 protein as a promising candidate for the differentiation of vaccinated versus infected and sero-diagnostic tools. The
current study elucidated the adjuvanticity effect of AgNP on IBDV rVP2 protein potency for the first time.
 
Date 2023-12-26T11:29:38Z
2023-12-26T11:29:45Z
2023-12-26T11:29:38Z
2023-12-26T11:29:45Z
2023-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63091
https://doi.org/10.56042/ijeb.v62i01.1609
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(01) [Jan 2024]