Record Details

DNA polymorphism in clonal and seedling progenies of an elite nutmeg (Myristica fragrans Houtt.) by RAPD

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator SHEEJA, T E
RAJESH, Y
KRISHNAMOORTHY, B
PARTHASARATHY, V A
 
Date 2002-07-27T13:43:32Z
2002-07-27T13:43:32Z
2006
 
Identifier Journal of plantation crops 34(3) 558 -561 (2006)
0304-5242
http://hdl.handle.net/123456789/196
 
Description Molecular profiling was done involving clonal and seedling progenies of a dwarf, high yielding, and high quality selection of nutmeg (Myristica fragrans Houtt.) very popular for its suitability to humid tropics. The RAPD profiles were analysed for detecting molecular variations and uniqueness in progenies. A total of 405 data points were scored with 20 polymorphic loci. The JSI values ranged from 0.72 to 1.00 showed the close relatedness among the progenies and mother. The clones were found to show definite variations from the mother. Clones No. 12, 15 and seedling No. 11 showed 100% similarity with the mother. The similarity index within clones (96-100%) was considerably more than that between clones and seedlings (83-100%) and within seedlings (76-90%). Twelve unique hands were detected for identification of individuals. The male seedling could be characterized by presence of specific bands of 1300 and 1000 bp amplifiable by OPC 16 and OPA 05 and absence of a band of 1400 and 1200 amplifiable by OPAOI and 710 by by OPA 14, respectively.
 
Format 569914 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Plantation Crops
 
Subject Myristica gragrans
RAPD
fingerprinting
gender identification
 
Title DNA polymorphism in clonal and seedling progenies of an elite nutmeg (Myristica fragrans Houtt.) by RAPD
 
Type Article