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Determination of amylose content of traditional rice varieties of Kerala using PCR-based molecular markers

Indian Agricultural Research Journals

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Field Value
 
Title Determination of amylose content of traditional rice varieties of Kerala using PCR-based molecular markers
 
Creator John, Deepa
Raman, Maya
 
Subject Rice
Wax allele
amylose content
molecular markers
 
Description The present study uses molecular approach to assess the amount of amylose in collected rice genotypes using PCR based markers. The polymorphism of Waxy allele which encodes the major enzyme catalyzing amylose synthesis was analyzed in the collected rice samples to determine the amylose content in the samples. All the rice varieties under study expressed Wxa allele corresponding to intermediate to high amylose content. This study proves that simple PCR technique can be used to detect Single nucleotide polymorphism mutation at Wx locus and provides a cost-effective alternative to predict amylose content in rice.
 
Publisher Association of Rice Research Workers
 
Date 2023-12-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Identifier https://epubs.icar.org.in/index.php/OIJR/article/view/146922
 
Source ORYZA-An International Journal of Rice; Vol. 60 No. 04 (2023): October-December; 508-513
2249-5266
0474-7615
 
Rights Copyright (c) 2023 Association of Rice Research Workers
http://creativecommons.org/licenses/by/4.0