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The Effect of short-term hyper-ammonia exposure in Indiancatfish, Clarias magur (Hamilton, 1822) on haematological parameters, tissues structure and gene expression profiles: Short term Ammonia exposure effects in catfish

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Title The Effect of short-term hyper-ammonia exposure in Indiancatfish, Clarias magur (Hamilton, 1822) on haematological parameters, tissues structure and gene expression profiles: Short term Ammonia exposure effects in catfish
 
Creator Kumar, Aditya
Mog Chowdhury, Labrechai
Rimal, Radhika
Kumar, Rajesh
Paul, Alisha
Faridi, Neha
Singh, Mridula
Singh, Achal
Mohindra, Vindhya
 
Description  In the present study, pattern of changes in blood physiological parameters, structuralchanges in gills, liver and kidnney as well as gene expression patterns for ornithine-ureacycle (OUC) were studied, under hyper-ammonia stress (25 mM NH4Cl) for 1, 3, 6 and 9 hand 24 h in normal water condition after 9 h exposure (9h+N), in Clarias magur (Hamilton,1822). Analysis showed increase in blood ammonia upto 3 h, with decreasing trend (upto2 folds reduction) subsequently. It was accompanied by simultaneous increase in urealevels upto 6 folds at 9 h exposure. However, there were no significant changes in bloodparameters, except for serum protein, serum glutamic pyruvic transaminase (SGPT) andlactate dehydrogenase (LDH) levels after 1 h exposure. Histological analysis of gills revealedhighest (88%) percentage of damaged gills with congested blood capillaries, treated for 9 h,but declined by 65% at 9h+N. Histological alterations in liver and kidney were prominentonly at 9 h exposure, with nuclear hypertrophy and necrosis of hepatic cells in liver tissuesand distension as well as degeneration of Bowman’s capsule in kidneys. Significantup-regulation of cpsIII and otc genes at 9 h and 9h+N, points to activation of OUC in liver at6 h exposure and unique physiological characteristics of turning towards ureotelism fromammoniotelism, after short term exposure to high ammonia level.
Keywords:Ammonium chloride; Clarias magur, Congested capillaries, Gills, Hyperplasia, Ornithine urea cycle, Urea
 
Publisher Central Marine Fisheries Research Institute (on behalf of Indian Council of Agricultural
 
Date 2023-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJF/article/view/142020
10.21077/ijf.2023.70.4.142020-12
 
Source Indian Journal of Fisheries; Vol. 70 No. 4 (2023)
0970-6011
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJF/article/view/142020/53534
 
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