In vitro propagation of Pyrus communis cv. Bartlett
Indian Agricultural Research Journals
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Title |
In vitro propagation of Pyrus communis cv. Bartlett
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Creator |
PADDER, M A
MIR, M A BHATT, K M RATHER, Z A HUSSAIN, G |
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Subject |
Bartlett
Forcing In vitro propagation Pyrus communis |
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Description |
Shoot tip explants derived from forced and unforced explants were subjected to different sterilization regimes. Satisfactory culture asepsis (61.60%) and survival (45.00%) was obtained with 0.1% mercuric chloride when forced explants were used. MS medium supplemented with BAP+IBA (1.5+0.01mg/l) resulted in maximum explant establishment (46.66%). Maximum proliferating cultures (52.66%) and shoot number/explant (8.73) was recorded in MS medium supplemented with BAP+IBA (2.00+0.01mg/l). Microcuttings (10-15mm) from the proliferated cultures were inoculated in two media supplemented with different IBA concentrations for rooting. MS medium fortified with IBA (1.0 mg/l) resulted in maximum rooting (46.66%), root number/shoot (4.26) and root length (36.50 mm).
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Publisher |
Indian Council of Agricultural Research
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Date |
2012-03-12
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion |
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Format |
application/pdf
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Identifier |
https://epubs.icar.org.in/index.php/IJAgS/article/view/15942
10.56093/ijas.v82i3.15942 |
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Source |
The Indian Journal of Agricultural Sciences; Vol. 82 No. 3 (2012); 225–9
2394-3319 0019-5022 |
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Language |
eng
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Relation |
https://epubs.icar.org.in/index.php/IJAgS/article/view/15942/7719
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Rights |
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0 |
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