Record Details

Characterization and bioactivity of β- ecdysterone from cell cultures of Trianthema decandra L.

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Characterization and bioactivity of β- ecdysterone from cell cultures of Trianthema decandra L.
 
Creator Pancholi, Boskey
 
Subject Antimicrobial
Antioxidant
Phytochemical analysis
Trianthema decandra
 
Description 617-623
In the present study, cell cultures from the nodal explants of Trianthema decandra L. were established on Murashige and
Skoog’s (MS) medium supplemented with different concentrations (0.5, 1, 1.5, and 2 mg/L) of indole 3-acetic acid (IAA),
2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kn). Induction of callus was promising in MS medium supplemented
with 1.5 mg/L 2,4-D after 25 days of inoculation. Their total phenolics and flavonoids levels were estimated and compared
with those of in vivo. Antimicrobial (agar well diffusion method) and antioxidant (DPPH and FRAP methods) activities
were studied. Further, six-weeks-old callus grown on MS medium with 2,4-D (1.5 mg/L) were harvested, dried and
extracted using absolute methanol. Methanolic extracts of callus showed maximum inhibition against B. subtilis, P.
aeruginosa, and A. flavus (IZ 14.33±0.32 mm, 15.66±0.32 mm and 13.33±0.65 mm, respectively) as compared to in vivo
plant extract. Similarly, in the case of DPPH activity, methanolic extract of the callus demonstrated more antioxidant
potential than in vivo. At 0.8 mg/mL concentration of callus extract showed inhibition of 85.02% as compared to 83.38% of
plants grown in vivo. Likewise, in the case of the FRAP method, there is no significant difference in antioxidant potential
(303±3.37 ascorbic acid equivalent/ mg in callus; AAE/mg) as compared to in vivo plant (310±10.01 AAE/mg). A new
compound β- ecdysterone (20-hydroxyecdysone), was isolated and identified from the callus of this plant, which was also
tested for its antimicrobial and antioxidant activity. Isolated pure compound β- ecdysterone were tested against selected
strains of bacteria and fungi and prove effective E. aerogenes and K. pneumoniae with Minimum inhibitory concentration of
250 μg/mL concentration (IZ 11.00±0.00 mm and 12.00±0.00). compound does not impressive for antifungal profile.
 
Date 2024-01-04T09:30:27Z
2024-01-04T09:30:27Z
2024-01
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/63156
https://doi.org/10.56042/ijnpr.v14i4.6167
 
Language en
 
Relation Int. cl. (2021.01)− A61K 36/00, A61P 31/00, A61P 39/00
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.14(4) [December 2023]