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Cytotoxic effects of fractions, isolates, and nanoparticles of soursop leaf (Annona muricata L.) and sappan wood (Caesalpinia sappan L.) against human cervical cancer HeLa cells

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Title Cytotoxic effects of fractions, isolates, and nanoparticles of soursop leaf (Annona muricata L.) and sappan wood (Caesalpinia sappan L.) against human cervical cancer HeLa cells
 
Creator Astirin, Okid Parama
Artanti, Anif Nur
Herawati, Elisa
Putri, Fitria Arayani
Muhyidin, Muhammad Fadli
 
Subject Annona muricata L
Caesalpinia sappan L.
HeLa cells
Nanoparticle
 
Description 524-532
This article compared the effectiveness of fractions, isolates, and nanoparticles form of soursop leaf (Annona muricata L.)
and sappan wood (Caesalpinia sappan L.) on inducing anti-proliferative and cytotoxic effects on cervical cancer HeLa cells.
Nanoparticles were formulated from the chloroform fraction of soursop leaf and the ethyl acetate fraction of sappan wood.
The cytotoxic effect of a single dose of fractions, isolates, and nanoparticles was tested using an MTT assay, while the
combined dose of nanoparticles was evaluated to determine the synergistic effect. Flow cytometry was used to evaluate
cancer cell death through apoptosis and necrosis, along with characterisation of the nanoparticles' morphology and size. The
MTT assay results showed that all forms of soursop leaf reduced the viability of HeLa cells (IC50 fraction 7.13 μg/mL,
isolate 8.17 μg/mL, nanoparticles 88.98 μg/mL) and sappan wood (IC50 fraction 13.07 μg/mL, isolate 6.9 μg/mL,
nanoparticles 82.53 μg/mL). However, the nanoparticle form of soursop leaf and sappan wood showed a higher IC50 value
than their fraction and isolate form. Flow cytometry test showed all forms of soursop leaf and sappan wood successfully
caused apoptosis and necrosis of HeLa cells. When tested in combined doses, soursop leaf and sappan wood nanoparticles
demonstrated a range of antagonistic, additive, and synergistic effects. The synergistic effect is evident at a combination of
1/10 IC50 of each nanoparticle with a combination index value of 0.54.
 
Date 2024-01-04T10:21:13Z
2024-01-04T10:21:13Z
2024-01
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/63166
https://doi.org/10.56042/ijnpr.v14i4.5779
 
Language en
 
Relation Int. cl. (2021.01)− A61K 36/00, A61K 36/48, A61K 127/00, A61K 135/00
 
Publisher NIScPR-CSIR,India
 
Source IJNPR Vol.14(4) [December 2023]