ICAR Research Data Repository for Knowledge Management
A simple modified method of DNA extraction from seeds for PCR amplifications
Indian Agricultural Research Journals
View Archive Info| Field | Value | |
| Title |
A simple modified method of DNA extraction from seeds for PCR amplifications
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| Creator |
GUPTA, RAMWANT
CHANDRASHEKAR, U S CHAKRABARTY, S K DADLANI, M |
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| Subject |
DNA
Genetic purity PCR Seed lots |
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| Description |
Rapid and reliable protocols for testing genetic purity of seed are an important requirement for quality assurance. This method was applied to Indian mustard, rice, wheat and cotton. Yield and quality of DNA was good for PCR analysis. DNA yield ranged from 16.4 to 76.22 ng/mg seed depending on plant species and varieties. The A260/280 ratio ranged from 1.65-1.91, indicating that the isolated DNA was fairly clean and free from protein and RNA contaminations. By adopting this method nearly 200–300 sample can be extracted in 24 hr, which will be useful for assessment of genetic purity of seed lots during seed multiplication as well as in breeding programmes that requires rapid screening of large population.
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| Publisher |
Indian Council of Agricultural Research
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| Date |
2012-01-03
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion |
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| Format |
application/pdf
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| Identifier |
https://epubs.icar.org.in/index.php/IJAgS/article/view/13882
10.56093/ijas.v82i1.13882 |
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| Source |
The Indian Journal of Agricultural Sciences; Vol. 82 No. 1 (2012); 75–7
2394-3319 0019-5022 |
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| Language |
eng
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| Relation |
https://epubs.icar.org.in/index.php/IJAgS/article/view/13882/6901
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| Rights |
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0 |
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