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Identification of a decapacitation factor in goat seminal plasma

Indian Agricultural Research Journals

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Title Identification of a decapacitation factor in goat seminal plasma
 
Creator KAUR, ARMINDER
SHARMA, G S
 
Subject Accessory glands
Capacitation
Decapacitation
Decapacitation factor (DF)
Seminal plasma
Sperm surface proteins
 
Description The seminal plasma is complex mixture of the secretions of the testis, epididymis and the accessory glands. The secretory proteins of the epididymal fluid as well as those of the accessory glands appear along with spermatozoa as semen at the time of ejaculation. Some of these proteins coat the sperm surface and prevent the premature activation of the spermatozoa in the female reproductive tract. The activation of spermatozoa, which requires the removal of sperm- coating proteins, is necessitated for the entry of the spermatozoa into the oocyte at the site of fertilization. The adsorption of radiolabelled seminal plasma proteins on the sperm surface showed an absence of high molecular weight proteins, thus suggesting the reorganization of the sperm surface proteins during the process of capacitation. The effect of lyophilized goat seminal plasma proteins revealed an inhibitory effect on the capacitation after an initial preincubation of 3.5 h. The absorption of radiolabelled seminal plasma proteins on the sperm surface showed an absence of high molecular weight proteins, thus suggesting the reorganization of the sperm surface proteins during the process of capacitation. The amount of protein for a 50% decrease in the acrosome reaction as compared to the control in a dose- dependent response, ID50 is 2.45 mg. The factor is removed by the help of ultracentrifugation for 6 h and the pellet obtained showed an inhibitory effect at an initial preincubation of 3.5 h and the value of ID50 determined is 1.25 mg. An apparent mechanism of capacitation involves the rearrangement of sperm surface proteins and removal of the factor by ultracentrifugation shows that it is a high molecular weight protein.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-12-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/25665
10.56093/ijans.v82i12.25665
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 12 (2012); 1504–1509
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/25665/11869
 
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