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PARTIAL CHARACTERIZATION OF THE VIRUS ASSOCIATED WITH FOORKEY DISEASE OF LARGE CARDAMOM (AMOMUM SUBULATUM ROXB.)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator SANGHAMITRA, V
 
Date 2008-08-13T07:09:52Z
2008-08-13T07:09:52Z
2008
 
Identifier http://hdl.handle.net/123456789/485
 
Description Virus causing foorkey disease of L. cardamom in India was partially characterized on the basis of Replicase gene nucleotide properties. A protocol for total DNA isolation from large cardamom infected with foorkey was standardized. Replicase gene of the virus was amplified using Polymerase Chain Reaction (PCR), cloned and sequenced. Sequenced region contain 552 nucleotides potentially coding for 184 amino acids. Sequence analysis with BBTV isolates showed an identity ranging from 92-99% and 93-99% at nucleotide and amino acid respectively. Since LC is showing maximum identity with BBTV isolates, it is considered as a strain of BBTV.
 
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Title PARTIAL CHARACTERIZATION OF THE VIRUS ASSOCIATED WITH FOORKEY DISEASE OF LARGE CARDAMOM (AMOMUM SUBULATUM ROXB.)
 
Type Thesis