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Screening of endophytic bacteria and evaluation of selected isolates for suppression of burrowing nematode (Radopholus similis Thorne) using three varieties of black pepper (Piper nigrum L.)

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator ARAVIND, R
EAPEN, S J
KUMAR, A
DINU, A
RAMANA, K V
 
Date 2010-03-08T07:21:16Z
2010-03-08T07:21:16Z
2010-04
 
Identifier Crop Protection No.29 :318-324 (2010)
0261-2194
http://hdl.handle.net/123456789/502
 
Description Radopholus similis is a serious threat to black pepper (Piper nigrum L.) cultivation being the main causal organism of slow decline disease. Because of its migratory nature most fungal and bacterial antagonists are ineffective in suppressing R. similis. The presence of a number of endophytic bacteria in balck pepper tissues has been proved in earlier studies. This study was undertaken to evaluate the bacteria isolated from black pepper fro suppressing R. similis. In vitro and in vivo screenings were used initially to identify the efficient strains of endophytic bacteria that suppress R. similis. Seventy four isolates of endophytic bacteria obtained from black pepper were screened against R. similis by various bioassays. Results of the in vitro experiments were inconclusive and did not match the rest of the studies. However, six isolates were shortlisted based on the preliminary in vivo screening and further tested in an evaluation trial using three varieties of black pepper. Irrespective of the varieties, significantly higher nematode suppression was observed with one isolate(TC 10) followed by another (BP 17). These isolates were identified to the species level by sequence analysis of the 16S rRNA gene. The results showed that these isolates shared 99% identity with Bacillus megaterium and Curtobacterium luteum, respectively. More studies are required to understand their mode of action as well as the dose-response relationship with nematodes.
 
Format 531687 bytes
application/pdf
 
Language en
 
Publisher Elsevier
 
Subject Biological Control
Black pepper
Burrowing Nematode
Endophytic bacteria
Piper nigrum
Radopolus similis
 
Title Screening of endophytic bacteria and evaluation of selected isolates for suppression of burrowing nematode (Radopholus similis Thorne) using three varieties of black pepper (Piper nigrum L.)
 
Type Article