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A novel approach for simultaneous differentiation of closely related meat species by PCR assay using single primer pair

Indian Agricultural Research Journals

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Title A novel approach for simultaneous differentiation of closely related meat species by PCR assay using single primer pair
 
Creator MANE, B G
MENDIRATTA, S K
TIWARI, A K
NARAYAN, RAJ
 
Subject Cattle, Differentiation, Heat treatment, Meat species, PCR assay, Sheep
 
Description The aim of the present study was to design the primer pair for differentiation of closely related meat species i.e. cattle from buffalo, and sheep from goat employing PCR assay. The primer pair was designed based on the mitochondrial 12S rRNA gene sequences of buffalo and then designed primer pair matched with other species available 12S rRNA gene sequences. The designed primer pair was successfully amplified to the desired DNA fragments of 316 bp from the buffalo and sheep in single PCR reaction assay without any cross reaction with cattle, goat, pig and chicken species. The developed PCR assay was able to differentiate the closely related meat species; however, this assay was not suitable for differentiation of buffalo from sheep species meat. This PCR assay was also effective in heat treated meat and meat emulsion without any adverse effect on amplification of DNA. It can be concluded that the developed PCR assay has potential application for the purpose of differentiation of closely related meat species.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-12-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/25697
10.56093/ijans.v82i12.25697
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 12 (2012); 1570–1573
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/25697/11885
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0