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Polymorphism of insulin like growth factor binding protein 3 (IGFBP3) partial gene in mithun

Indian Agricultural Research Journals

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Title Polymorphism of insulin like growth factor binding protein 3 (IGFBP3) partial gene in mithun
 
Creator PANIGRAHI, MANJIT
KUMAR, SUBODH
DEB, S M
MITRA, ABHIJIT
CHOWDHARY, HITU
SHARMA, ARJAVA
 
Subject Bos frontalis
exon 2–3
Hinf I
IGFBP-3
Mithun
PCR-RFLP
 
Description A fragment of 652 bp corresponding to exon–2–3 of IGFBP3 gene was amplified in 90 mithun (Bos frontalis). The PCR- RFLP analysis showed the absence of polymorphism in this fragment with respect to HinfI restriction enzyme and showed only 2 restriction sites, which produced 3 fragments of 314, 206 and 132 bp (AA genotype). Six single nucleotide differences viz. T34C, C164G, T175C, A354G, T599C and G614A existed in mithun sequences when compared to cattle. The neocleotide and amino acid sequence homology between them was 98.2 and 100% respectively. Mithun had minimum deviation from cattle with respect to nucleotide as well as amino acid sequences. The sequence of the amplicon, which was the first report on mithun IGFBP3, was submitted to GenBank (Accession number EF686017).
 
Publisher Indian Council of Agricultural Research
 
Date 2012-12-13
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/25700
10.56093/ijans.v82i12.25700
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 12 (2012); 1584–1586
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/25700/11888
 
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