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Anther-derived microspore embryogenesis in pepper hybrids orobelle and Bomby

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Title Anther-derived microspore embryogenesis in pepper hybrids orobelle and Bomby
 
Creator K. P. Sahana
Arpita Srivastava
Anil Khar
Neelu Jain
P. K. Jain
Hemlata Bharti
Mohd Harun
Manisha Mangal
 
Subject Anther-derived microspore embryogenesis
Bomby
Orobelle
 
Description Not Available
Background
Traditional breeding methods have long been employed worldwide for the evaluation and development of pepper cultivars. However, these methods necessitate multiple generations of screening, line development, evaluation, recognition, and crossing to obtain highly homozygous lines. In contrast, in vitro anther-derived microspore culture represents a rapid method to generate homozygous lines within a single generation. In the present study, we have optimized a protocol for microspore embryogenesis from anther cultures of pepper hybrids Orobelle and Bomby.

Results
We achieved early and successful embryo formation from both genotypes by subjecting the buds to a cold pretreatment at 4 °C for 4 days. Our optimized culture medium, comprised of MS medium supplemented with 4 mg/L NAA, 1 mg/L BAP, 0.25% activated charcoal, 2.6 g/L gelrite, 30 g/L sucrose, and 15 mg/L silver nitrate, exhibited the highest efficiency in embryo formation (1.85% and 1.46%) for Orobelle and Bomby, respectively. Furthermore, successful plant regeneration from the anther derived microspore embryos was accomplished using half-strength MS medium fortified with 2% sucrose and 0.1 mg/L 6-benzylaminopurine (BA), solidified with 2.6 g/L gelrite. The ploidy status of the microspore-derived plantlets was analyzed using flow cytometry technique. Notably, the haploid plants exhibited distinct characteristics such as reduced plant height, leaf length, leaf width, and shorter internode length when compared to their diploid counterparts derived from seeds.

Conclusion
Our findings highlight the potential of anther culture and microspore embryogenesis as an advanced method for accelerating pepper breeding programs, enabling the rapid production of superior homozygous lines.
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Date 2024-01-12T16:01:42Z
2024-01-12T16:01:42Z
2024-01-04
 
Type Research Paper
 
Identifier Sahana, K.P., Srivastava, A., Khar, A. et al. Anther-derived microspore embryogenesis in pepper hybrids orobelle and Bomby. Bot Stud 65, 1 (2024). https://doi.org/10.1186/s40529-023-00408-6
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http://krishi.icar.gov.in/jspui/handle/123456789/81249
 
Language English
 
Relation Not Available;
 
Publisher Not Available