Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus
DSpice at Indian Institute of Spices Research
View Archive InfoField | Value | |
Creator |
BHAT, A I
SILJO, A JIBY, M V THANKAMANI, C K MATHEW, P A |
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Date |
2010-08-10T08:29:18Z
2010-08-10T08:29:18Z 2009 |
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Identifier |
Journal of Spices and Aromatic Crops Vol. 18 (1) : 28-32 (2009)
0971-3328 http://hdl.handle.net/123456789/538 |
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Description |
A polymerase chain reaction (PCR) based method was used for indexing 845 plants representing 14 popular varieties of black pepper (Piper nigrum) showing no visible external symptoms (apparently healthy) for Piper yellow mottle virus (PYMoV), among which 694 plants were positive (82%) for PYMoV. The percentage of infected plants ranged from 59% to 100% in different varieties; 100% of the tested plants of Panniyur-6 were infected with PYMoV and Sreekara had the least number of infected plants (59%). Some of the indexed PCR positive plants exhibited visible symptoms such as mild chlorosis, yellow specks and mottling in 1-3 months after testing. The study showed that indexing by PCR successfully detected PYMoV in infected black pepper plants showing no visible symptoms. The method can be used in certification programmes to identify PYMoV-free plants for further propagation.
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Format |
386167 bytes
application/pdf |
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Language |
en
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Publisher |
Indian Society for Spices
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Subject |
Black pepper
indexing PCR Piper nigrum Piper yellow mottle virus |
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Title |
Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus
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Type |
Article
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