Record Details

Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator BHAT, A I
SILJO, A
JIBY, M V
THANKAMANI, C K
MATHEW, P A
 
Date 2010-08-10T08:29:18Z
2010-08-10T08:29:18Z
2009
 
Identifier Journal of Spices and Aromatic Crops Vol. 18 (1) : 28-32 (2009)
0971-3328
http://hdl.handle.net/123456789/538
 
Description A polymerase chain reaction (PCR) based method was used for indexing 845 plants representing 14 popular varieties of black pepper (Piper nigrum) showing no visible external symptoms (apparently healthy) for Piper yellow mottle virus (PYMoV), among which 694 plants were positive (82%) for PYMoV. The percentage of infected plants ranged from 59% to 100% in different varieties; 100% of the tested plants of Panniyur-6 were infected with PYMoV and Sreekara had the least number of infected plants (59%). Some of the indexed PCR positive plants exhibited visible symptoms such as mild chlorosis, yellow specks and mottling in 1-3 months after testing. The study showed that indexing by PCR successfully detected PYMoV in infected black pepper plants showing no visible symptoms. The method can be used in certification programmes to identify PYMoV-free plants for further propagation.
 
Format 386167 bytes
application/pdf
 
Language en
 
Publisher Indian Society for Spices
 
Subject Black pepper
indexing
PCR
Piper nigrum
Piper yellow mottle virus
 
Title Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus
 
Type Article