Record Details

Genome Wide Association Mapping of Net Form Net Blotch resistance in Barley reveals multiple disease resistance loci for resistance to biotrophic and necrotrophic pathogens

MELSpace

View Archive Info
 
 
Field Value
 
Title Genome Wide Association Mapping of Net Form Net Blotch resistance in Barley reveals multiple disease resistance loci for resistance to biotrophic and necrotrophic pathogens
 
Creator Rehman, Sajid
 
Contributor Al-Jaboobi, Muamar
Al-Abdallat, Ayed
Verma, Ramesh Pal Singh
Sanchez-Garcia, Miguel
Visioni, Andrea
 
Subject net blotch
genome wide association
 
Description The net form net blotch (NFNB) caused by Pyrenophora teres f. teres (Ptt) is an emerging barley
disease in several countries. It causes severe yield and quality losses due to infection of leaves, kernels,
and stems. Owing to inherent genetic diversity of Ptt, the incorporation of qualitative and quantitative
resistance is important to obtain barley cultivars with durable resistance to NFNB. For this purpose, an
association mapping panel named as HI-AM (High Input Association Mapping) was screened for
resistance to NFNB at the seedling stage with two virulent Moroccan Ptt (Ptt40-3, Ptt45-3) under
controlled conditions, and at the adult plant stage at four hot spot locations in Morocco during different
cropping seasons (2016-17 and 2017-18). Genome wide association mapping (GWAM) was conducted
using 13,182 PAV ((presence or absence variations) and 6,311 SNP markers (DaRTSeq) for mapping of
seedling and adult plant resistance quantitative trait loci (QTL). GWAM analysis revealed 19 QTL for
seedling stage and 35 QTL for adult plant stage resistance. Of the 54 QTL detected, our study validated
38 QTL already reported in literature while 16 QTL were novel. Seedling stage QTL explained together
40% of the genetic variance for seedling resistance to isolate Ptt40, and 69% for isolate Ptt45, whereas
the genetic variance of QTL for adult plant stage resistance ranged from 35% to 85%. This panel was
previously used for other GWAM studies including resistance to spot blotch and stripe rust of barley. By
mapping of significant markers for three different diseases on the Morex genome version 3, we also
identified 13 common QTL associated with resistance to net blotch and spot blotch and 3 QTL associated
with resistance to all three disease. The identification and introgression of common QTL conditioning
resistance to three diseases could help in attaining durable disease resistance to three major diseases of
barley in North Africa.
 
Date 2024-01-16T17:59:01Z
2024-01-16T17:59:01Z
 
Type Conference Paper
 
Identifier https://apbaconf2023.um6p.ma/conference-book.pdf
https://mel.cgiar.org/reporting/download/hash/e853f1e32818a1a145ccb77ee9dd4b0d
Sajid Rehman, Muamar Al-Jaboobi, Ayed Al-Abdallat, Ramesh Pal Singh Verma, Miguel Sanchez-Garcia, Andrea Visioni. (10/10/2023). Genome Wide Association Mapping of Net Form Net Blotch resistance in Barley reveals multiple disease resistance loci for resistance to biotrophic and necrotrophic pathogens.
https://hdl.handle.net/20.500.11766/69044
Open access
 
Language en
 
Rights Copyrighted; Non-commercial educational use only
 
Format PDF