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Molecular detection of Dirofilaria immitis in dogs

Indian Agricultural Research Journals

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Title Molecular detection of Dirofilaria immitis in dogs
 
Creator Wasan A Alobaidii
Shahad Abbas Ali
 
Subject Dirofilariaimmitis , Dogs,Polymerase Chain Reaction,Mosul
 
Description There are different species of nematodes which affect dogs health but the Dirofilariaimmitisis the dangerous one , in this study2 ml of blood from one hundred  four  of samples, from dogs different  breeds,breeding managements  and age range from less than 6 months  to more than 4 years old , then DNA extracted from this blood , PCR technique using a Primer designed as (  5-AGTGTAGGTCAGCCCTGAGTTA-3 ) forward and ( 5-ACAGGCACTGACAATACCAAT-3 ) was used to amplify 203 bp in mitochondrial oxidase (COI), COI gene amplification results showed 203 bp of band for 15 blood samples.not Anyof the negative samples for D.immitis give amplification of the DNA molecules, 15 samples from 104 animalsshowed  positive using PCR (14.4%) ,high prevelance of infection in  dogs 2-4years old (40%) , while the animals  less than one year old not recorded any infection rates, Outdoor animals showed high prevalence of infection with D.immitis when compare with indoor once
 
Publisher Indian Veterinary Association
 
Date 2024-01-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IVJ/article/view/147601
10.62757/IVA.2024.101.1.7-11
 
Source The Indian Veterinary Journal; Vol. 101 No. 01 (2024): January, 2024; 7-11
0974-9365
0019-6479
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IVJ/article/view/147601/53681
 
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