Record Details

Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/12390/
https://acsess.onlinelibrary.wiley.com/doi/10.1002/tpg2.20337
https://doi.org/10.1002/tpg2.20337
 
Title Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)
 
Creator Kudapa, H
Ghatak, A
Barmukh, R
Chaturvedi, P
Khan, A
Kale, S
Fragner, L
Chitikineni, A
Weckwerth, W
Varshney, R K
 
Subject Chickpea
Drought
 
Description Drought is one of the major constraints limiting chickpea productivity. To unravel complex mechanisms regulating drought response in chickpea, we generated transcriptomics, proteomics, and metabolomics datasets from root tissues of four contrasting drought-responsive chickpea genotypes: ICC 4958, JG 11, and JG 11+ (drought-tolerant), and ICC 1882 (drought-sensitive) under control and drought stress conditions. Integration of transcriptomics and proteomics data identified enriched hub proteins encoding isoflavone 4′-O-methyltransferase, UDP-d-glucose/UDP-d-galactose 4-epimerase, and delta-1-pyrroline-5-carboxylate synthetase. These proteins highlighted the involvement of pathways such as antibiotic biosynthesis, galactose metabolism, and isoflavonoid biosynthesis in activating drought stress response mechanisms. Subsequently, the integration of metabolomics data identified six metabolites (fructose, galactose, glucose, myoinositol, galactinol, and raffinose) that showed a significant correlation with galactose metabolism. Integration of root-omics data also revealed some key candidate genes underlying the drought-responsive “QTL-hotspot” region. These results provided key insights into complex molecular mechanisms underlying drought stress response in chickpea.
 
Publisher Crop Science Society of America
 
Date 2023-05-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by_nc_nd
 
Identifier http://oar.icrisat.org/12390/1/The%20Plant%20Genome_1-17_2023.pdf
Kudapa, H and Ghatak, A and Barmukh, R and Chaturvedi, P and Khan, A and Kale, S and Fragner, L and Chitikineni, A and Weckwerth, W and Varshney, R K (2023) Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.). The Plant Genome (TSI), SE. pp. 1-17. ISSN 1940-3372