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Application of loop mediated isothermal amplification (LAMP) assay as an alternative diagnostic test for rapid tuberculosis diagnosis in limited resource setting

Indian Agricultural Research Journals

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Title Application of loop mediated isothermal amplification (LAMP) assay as an alternative diagnostic test for rapid tuberculosis diagnosis in limited resource setting
 
Creator DOLKER, SONAM
VERMA, RISHENDRA
PRAKASH, CHANDAN
SHAMAL, ASHABREE
 
Subject Mycobacterium tuberculosis
LAMP
TB diagnosis
 
Description Diagnostic potentiality of loop mediated isothermal amplification (LAMP) assay with human sputum samples was compared with smear microscopy, culture and PCR in providing rapid and accurate TB diagnosis. Practical application of this assay was emphasized as a supplementary diagnostic test with acid fast smear microscopy for controlling tuberculosis in limited resource setting particularly India. Sputum samples (261) from TB suspected patients were collected, processed and subjected to smear microscopy, culture, PCR and LAMP assay. These 4 diagnostic methodologies were compared in terms of sensitivity, specificity, and rapidity and user friendliness. Sensitivity of LAMP, PCR and microscopy were 97.47, 97.47, and 61.61%, respectively, and specificity of LAMP, PCR and microscopy were 60.31, 74.60 and 100% respectively. Higher sensitivity of diagnostic test will be more useful in high TB endemicity area to cover most of TB patients under National Tuberculosis Control Programmae. This LAMP assay may be a viable alternative of costly PCR diagnosis. This test may be adopted as supplementary diagnostic test with acid fast smear microscopy for routine diagnosis of human TB.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-11-20
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/25129
10.56093/ijans.v82i11.25129
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 11 (2012); 1293–1297
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/25129/11690
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0