Record Details

Endogenous and exogenous bone morphogenetic protein-2 play an important role in chondrocytic differentiation and maturation in rat cord blood-derived mesenchymal progenitor cells

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Endogenous and exogenous bone morphogenetic protein-2 play an important role in chondrocytic differentiation and maturation in rat cord blood-derived mesenchymal progenitor cells
 
Creator UCHIDA, KENTARO
NARUSE, KOUJI
UENO, MASAKI
ONUMA, KENJI
URABE, KEN
SAKAI, RINA
MORIYA, MITSUTOSHI
ITOMAN, MORITOSHI
TAKASO, MASASHI
 
Subject Bone morphogenetic protein-2
Chondrogenesis
Mesenchymal progenitor cells
Rat cord blood
 
Description Bone morphogenetic protein-2 (BMP-2) strongly induces chondrogenesis in the presence of transforming growth factor beta-1 (TGF-β1) or –3 in bone marrow (BM), synovium, adipose, and muscle-derived mesenchymal stem cells (MSCs). However, the effect of BMP-2 on umbilical cord derived (UCB)-derived MSCs, which are potentially useful for cartilage repair, has not been elucidated. In this study, the effect of endogenous and exogenous BMP-2 on rat umbilical cord blood-derived mesenchymal progenitor cells (UCB-MPCs) was evaluated. When cells were cultured in medium supplemented with only 10% FBS, type II procollagen (COL2A1) transcripts and collagen II protein were detectable after 2 weeks in UCB-MPCs, but not in BM-MPCs. The BMP antagonist, noggin, suppressed gene expression of COL2A1 and SOX-9 in UCB-MPCs. Exogenous BMP-2 promoted chondrogenic differentiation of UCB-MPCs in a dose-dependent manner, even in the absence of exogenous TGF-b1. UCB-MPCs expressed both type IIA and IIB procollagen in medium supplemented with only 10% FBS. Notably, exogenous BMP-2 was able to stimulate COL2A1 expression, particularly that of the type IIB transcripts, and also induce chondrogenic differentiation in rat UCB-MPCs. Interestingly, TGF-β1 inhibited BMP-2-induced COL2A1 splicing. As our results suggest that exogenous BMP-2 plays an important role for the spontaneous differentiation of chondrogenic lineages in rat UCB-MPCs, exogenous BMP-2 may effectively promote cartilage repair when UCB-MPCs are used.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-11-20
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/25140
10.56093/ijans.v82i11.25140
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 11 (2012); 1335–1339
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/25140/11699
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0