Record Details

Multiplex PCR for rapid detection of Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae in subclinical mastitis milk

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Multiplex PCR for rapid detection of Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae in subclinical mastitis milk
 
Creator SHOME, BIBEK RANJAN
BHUVANA, MANI
MITRA, SUSWETA DAS
KRITHIGA, NATESAN
SHOME, RAJESWARI
VELU, DHANIKACHALAM
BANERJEE, APALA
SENGUPTA, PINAKI PRASAD
RAHMAN, HABIBAR
 
Subject Bovine mastitis
Diagnosis
Mastitis
Multiplex PCR
Streptococci
 
Description To improve mastitis diagnosis and achieve rapid, specific, reliable and cost effective test, a multiplex PCR for simultaneous detection and differentiation of major streptococcal species, viz. Streptococcus agalactiae, Streptococcus uberis and Streptococcus dysgalactiae was developed. Evaluation with 24 ATCC strains and 606 strains comprising streptococci (84) and staphylococci (522) showed the assay to be highly accurate. The threshold of detection of the mPCR assay was 10fg of genomic DNA and < 102 CFU ml-1. Assessment of 115 milk samples collected from subclinically infected herd, showed mPCR assay to be more efficacious than culture method. Identification of Streptococcus species using this assay will be crucial to determine prevalence of Streptococcus in a herd. This will facilitate early diagnosis, treatment and control of the rate of infection at farm level. The assay can be implemented for routine monitoring of herd health and can be of great value for promoting prevention of streptococcal mastitis.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-10-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/24279
10.56093/ijans.v82i10.24279
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 10 (2012); 1137–1141
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/24279/11511
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0