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Assessment of in-vivo safety and antibacterial activity of phages against methicillin resistant S. aureus in mouse model

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Title Assessment of in-vivo safety and antibacterial activity of phages against methicillin resistant S. aureus in mouse model
 
Creator Shukla, Sanjay
Nayak, Anju
Sharma, R K
Singh, A P
Singh, R V
Gupta, B
 
Subject Bacteriophage
Methicillin resistance
Mice
Phage lysate
Staphylococcus aureus
 
Description 83-87
Traditionally Ganges water that contains bacteriophages, are used in Hindu rituals in India. Bacteriophages are
bacterial-specific viruses and present abundantly on Earth. They are present most widely in the biosphere, soil and the
intestine of animals. Specificity of infection makes them a powerful alternative for the control of bacterial infection which is
normally showing resistant to antibiotics. In present study, isolation of bacteriophage was carried out from sewage of
piggery and dairy farms to find out antimicrobial property. Isolated bacteriophages were showing an icosahedral symmetry
with long tail of 109 nm and head of 52.20 nm in diameter under electron microscopic observation. These phages were
considered as member of Myoviridae family. Presently, antimicrobial resistance is an acute problem across the globe, and
therefore alternate for antimicrobials should be searched on priority. Primary aim of this study was to isolate and evaluate
the efficacy of a bacteriophage against Multi drug resistance pathogen like Methicillin resistance Staphylococcus aureus.
A total 150 sewage samples were processed for bacteriophage isolation out of them 27 were found to be positive for
bacteriophages. Four isolates of bacteriophage lysate ØVS1, ØVS5, ØVS9 and ØVS 27 were used as a cocktail in trail.
Therapeutic and safety profile of the purified phage lysate cocktail were performed in well-defined mouse model
at laboratory animal house of College of Veterinary Science & A.H., Jabalpur (M.P.). Results showed 100% in mice
G-III (challenged with MRSA) was treated with phage Cocktail with in ten days whereas G-IV was showing approx. 40% in
abscess (challenged with MRSA). Our findings reported that the bacteriophage therapy is safe and better than traditional
antibiotics therapy especially in multidrug resistance cases.
 
Date 2024-01-30T09:28:25Z
2024-01-30T09:28:25Z
2024-01
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/63239
https://doi.org/10.56042/ijtk.v23i1.8150
 
Language en
 
Relation Int Cl.24: C12N 7/00, C12R 1/445
 
Publisher NIScPR-CSIR, India
 
Source IJTK Vol.23(1) [January 2023]