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Inhibition of Bungarus caeruleus snake venom toxicity by Citrus reticulata methanolic extract and in-silico analysis of possible binding modes

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Title Inhibition of Bungarus caeruleus snake venom toxicity by Citrus reticulata methanolic extract and in-silico analysis of possible binding modes
 
Creator Raghavan, Srimathi
Jayaraman, Gurunathan
 
Subject Snakebite
Citrus aurantium
Citrus reticulata
Venom
Neutralization
Bungarus caeruleus
 
Description 67-76
Snakebite envenomation has been one of the major causes of morbidity and mortality around the world. Though intravenous administration of commercial anti-venom systemically neutralizes the toxicity, adverse effects are commonly seen in the post-treatment period. Plant metabolites and their derivatives have been shown to exhibit a wide range of anti-ophidian properties. In this study, we have investigated the Bungaruscaeruleus venom neutralization potential of Citrus aurantium and Citrus reticulata Blanco peels in terms of phospholipase and hemolytic inhibition. Methanolic extract of C. reticulata exhibited the highest inhibition (93%) of PLA2 activity. However, the extracts of both these Citrus species significantly inhibited the hemolytic activity of the venom (54–82%). Molecular docking indicated the binding of the citrus metabolites to catalytic site residues (TRP19, GLY30, TYR31, GLY32, and HIS48) of PLA2. In vivo studies using Swiss Albino mice confirmed the neutralising capacity of the citrus peel extract, as reflected by the increase in survival time. Therefore, the study reveals that these metabolites in the methanolic extract of the citrus peel can be used as supplements for the management of snake bites.
 
Date 2024-01-30T09:35:32Z
2024-01-30T09:35:32Z
2024-01
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/63241
https://doi.org/10.56042/ijtk.v23i1.8147
 
Language en
 
Relation Int Cl.24: A61K 36/752, A61K 38/17
 
Publisher NIScPR-CSIR, India
 
Source IJTK Vol.23(1) [January 2023]