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Evaluation of genetic fidelity of in vitro regenerated Sweet flag (Acorus calamus L.) using molecular markers

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Title Evaluation of genetic fidelity of in vitro regenerated Sweet flag (Acorus calamus L.) using molecular markers
 
Creator Purushottam, Paraskar Shyam
B, Kishor
Meetei, Ng. Tombisana
 
Subject Monomorphic amplified bands
Random amplified polymorphic DNA (RAPD)
 
Description 133-139
Sweet flag (Acorus calamus L.) is an endangered, perennial wild herb known for its high medicinal properties. An efficient in vitro regeneration protocol for sweet flag was developed and the genetic fidelity was assessed across in vitro regenerants. Murashige & Skoog media fortified with different plant growth regulators (PGRs) in various concentrations and combinations were used to culture rhizome buds. For surface sterilization of explants, treatment of fungicide (0.2% Bavistin) for duration of 30 min followed by treatment with 70% ethanol for 30 s and mercuric chloride (0.1%) for 15 min was found most effective. MS solid media (0.8% agar) was found more promising than MS semisolid media (0.6% agar) in relation to explants establishment. The maximum number of shoots per explant was observed in solid MS medium supplemented with 15 μM thidiazuron (TDZ) with an average of 7.67 shoots per explants and average explant response of 60%. Shoot length response was maximum in MS containing 10 μM 6-benzylaminopurine (BAP) in combination with 5 μM 1-naphthalene acetic acid (NAA) with an average length of 6.88 cm and percentage explant response of 80%. For rootinduction, MS medium containing 10 μM indole-3-butyric acid (IBA) gave the maximum number of roots (6.50) and rootlength (3.50 cm). Plants acclimatized well with 57.14% survivability rate. Forty random amplified polymorphic DNA(RAPD) primers were used to evaluate genetic fidelity among in vitro regenerated plants, one from each treatment andcompared with mother plant. Out of them, 20 RAPD primers produced a total of 70 fragments ranging from 350-2500 bp.The amplified bands of all in vitro plant samples and mother plant were observed to be monomorphic. The results showedthat the in vitro regenerated plants were genetically stable.
 
Date 2024-01-31T09:32:05Z
2024-01-31T09:32:05Z
2024-02
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/63273
https://doi.org/10.56042/ijeb.v62i02.3738
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.62(02) [Feb 2024]