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Improved humoral immune response of oil adjuvant vaccine by saponin co-adjuvantation against haemorrhagic septicaemia in mice and buffalo calves

Indian Agricultural Research Journals

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Title Improved humoral immune response of oil adjuvant vaccine by saponin co-adjuvantation against haemorrhagic septicaemia in mice and buffalo calves
 
Creator Kumar, Sujeet
Chaturvedi, V K
Kumar, B
Kumar, P
Somarajan, S R
Kumar, A
Yadav, A S
Sharma, B
 
Subject Buffalo
Co-adjuvantation
Haemorrhagic septicaemia
Oil adjuvant vaccine
Pasteurella multocida' Saponin
 
Description Oil adjuvant vaccine (OAV) formulations, viz. OAV (OAV without saponin) and S-OAV (OAV with 420 µg saponin/ ml) were prepared against haemorrhagic septicaemia using Pasteurella multocida, P52 antigen. Three groups of mice, each group with 40 mice, were inoculated with respective OAV, S-OAV and sterile phosphate buffer saline and monitored the ELISA antibody titer for 45 days. In another experiment, 2 groups of buffalo calves, each group with 3 calves, were vaccinated with OAV and S-OAV and monitored the ELISA antibody titer for 4.5 months. Viscosity of OAV was 222.28 and of S-OAV 215.10 centipoise. The anti-P. multocida specific serum ELISA antibody titers were significantly higher in S-OAV on 14th days post vaccination compared to OAV and control and remained consistently higher during the experiment. Fluorescent activated cell sorter study revealed that CD4 T cells were significantly higher in S-OAV group as compared to OAV and control, while CD8 T cells was 28.83% higher than control. All vaccinated groups were 100% protected after direct intraperitoneal challenge with 100 LD50 of live P. multocida, serotype B, strain P52 on 45th DPV. However, after challenge with 1000 LD50 the S-OAV group had 80% protection as compared to 60% protection by OAV. Trial on buffalo calves further confirmed the finding of mice model as higher ELISA antibody titers were observed by S-OAV group as compared to OAV throughout the experiment period since seventh DPV onwards. Thus, saponin co- adjuvantation of OAV increased the CD4, CD8 T cells population, improved the humoral immune response and protection.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-09-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/23644
10.56093/ijans.v82i9.23644
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 9 (2012); 953–957
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/23644/11328
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0