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Comprehensive Transcriptome Profiling Uncovers Molecular Mechanisms and Potential Candidate Genes Associated with Heat Stress Response in Chickpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/12413/
https://www.mdpi.com/1422-0067/24/2/1369
https://doi.org/10.3390/ijms24021369
 
Title Comprehensive Transcriptome Profiling Uncovers Molecular Mechanisms and Potential Candidate Genes Associated with Heat Stress Response in Chickpea
 
Creator Kudapa, H
Barmukh, R
Garg, V
Chitikineni, A
Samineni, S
Agarwal, G
Varshney, R K
 
Subject Chickpea
Legume Crops
 
Description Chickpea (Cicer arietinum L.) production is highly susceptible to heat stress (day/night temperatures above 32/20 °C). Identifying the molecular mechanisms and potential candidate genes underlying heat stress response is important for increasing chickpea productivity. Here, we used an RNA-seq approach to investigate the transcriptome dynamics of 48 samples which include the leaf and root tissues of six contrasting heat stress responsive chickpea genotypes at the vegetative and reproductive stages of plant development. A total of 14,544 unique, differentially expressed genes (DEGs) were identified across different combinations studied. These DEGs were mainly involved in metabolic processes, cell wall remodeling, calcium signaling, and photosynthesis. Pathway analysis revealed the enrichment of metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction, under heat stress conditions. Furthermore, heat-responsive genes encoding bHLH, ERF, WRKY, and MYB transcription factors were differentially regulated in response to heat stress, and candidate genes underlying the quantitative trait loci (QTLs) for heat tolerance component traits, which showed differential gene expression across tolerant and sensitive genotypes, were identified. Our study provides an important resource for dissecting the role of candidate genes associated with heat stress response and also paves the way for developing climate-resilient chickpea varieties for the future.
 
Publisher MDPI
 
Date 2023-01-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by
 
Identifier http://oar.icrisat.org/12413/1/International%20Journal%20of%20Molecular%20Sciences_24_2_1-20_2023.pdf
Kudapa, H and Barmukh, R and Garg, V and Chitikineni, A and Samineni, S and Agarwal, G and Varshney, R K (2023) Comprehensive Transcriptome Profiling Uncovers Molecular Mechanisms and Potential Candidate Genes Associated with Heat Stress Response in Chickpea. International Journal of Molecular Sciences (TSI), 24 (2). pp. 1-20. ISSN 1422-0067