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Assessment of immunolocalization of Fas antigen in the ovarian follicle and the role of IGF-I in Fas-L induced granulosa cell apoptosis in water buffalo

Indian Agricultural Research Journals

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Title Assessment of immunolocalization of Fas antigen in the ovarian follicle and the role of IGF-I in Fas-L induced granulosa cell apoptosis in water buffalo
 
Creator Patil, Sunil Kumar
Ravindra, J P
Jamuna, K V
Joseph, Dhanya
Raghvendra, B S
Prasad, R V
Selvaraju, S
 
Subject Buffalo
Fas
IGF-I
Granulosa cells
Immunohistochemistry
 
Description Primordial follicle pool size in the ovary is small and rate of follicular atresia is high in water buffaloes. The objective of this experiment was to study the expression of Fas antigen in granulosa cells of water buffalo ovarian follicle, and to know the role of insulin like growth factor-1(IGF-I) on Fas-ligand (Fas-L) mediated apoptosis. The Fas expression was observed mainly in the granulosa cells of the follicles. The expression was higher in the granulosa cells, which were denuded from basement membrane, indicating apoptosis. Healthy follicles which had intact multilaminar granulosa cell layer showed negative reaction for Fas antigen. The granulosa cells from 3–6 mm diameter follicles of the buffalo ovaries collected from the slaughterhouse ovaries were cultured in vitro to assess the effect of Fas-L at 10, 20 and 50ng/ ml on granulosa cell viability. The Fas-L even at the lowest dose tested (10ng/ml) significantly decreased granulosa cells viability as compared to control; whereas addition of IGF-I to the culture significantly improved granulosa cell viability. Addition of IGF-I could significantly improve the granulosa cells viability in the Fas-L treated culture. Overall this study demonstrated the existence of Fas and Fas mediated apoptosis in the granulosa cells of the buffalo follicle, and also suggested that IGF-I could rescue granulosa cells from Fas-L mediated apoptosis.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-09-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/23649
10.56093/ijans.v82i9.23649
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 9 (2012); 979–983
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/23649/11333
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0