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Expression profile of ghrelin and ghrelin receptor in cyclic goat ovary

Indian Agricultural Research Journals

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Title Expression profile of ghrelin and ghrelin receptor in cyclic goat ovary
 
Creator Chandra, Vikas
Ram, Hira
Sharma, A K
 
Subject Ghrelin
Goat
mRNA expression
Ovary
Receptor
 
Description Ghrelin, a 28-amino acid acylated peptide, was identified as the endogenous ligand for the GH secretagogue receptor and recently the role of ghrelin in reproduction was established. Total RNA was extracted from corpus luteum (CL) of the slaughterhouse derived goat ovaries of different stages of cyclicity. First strand cDNA synthesis was done using constant quantity of total RNA (8µg / 20 µl reaction mixture) and M-MuLV-RT at 36°C for 1h. Semi quantitative PCR analysis as well as real time PCR (Q-PCR) analysis revealed, persistent expression of ghrelin and its receptor throughout the estrous cycle. Ghrelin mRNA corresponded the activity of CL i.e. minimum expression in the degenerating CL and it was at peak in the fully grown CL and similar trend was also observed for ghrelin receptor. Further, ghrelin and its receptor was absent in prepubertal ovary while it was present in cyclic goat ovaries. It can be concluded that ghrelin may have role in the regulation of ovarian follicular development and early embryonic development.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-09-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/23650
10.56093/ijans.v82i9.23650
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 9 (2012); 984–987
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/23650/11334
 
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