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Biochemical characterization of foot-and-mouth disease virus

Indian Agricultural Research Journals

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Field Value
 
Title Biochemical characterization of foot-and-mouth disease virus
 
Creator Nath, Rita
Prasad, Ram Lakhan
Sarma, Sankar
 
Subject FMD
Mobility
Molecular weight
Protein
Vaccine
 
Description The present study was carried out to determine the molecular weight and mobility of the proteins present in the FMD virus. FMD trivalent vaccine and FMD infected serum samples were used for the study. The standard SDS-6H of known proteins was used for calculating the molecular weight of different proteins.The molecular weight of the FMD viral proteins 1 and 2 were 101 and 62 K Da and mobilities were 0.268 and 0.521 respectively. Seven distinct bands segregrated in control and FMD affected serum samples. The bands in the control sample from the top of the gel were identified as g globulin, ß globulin, unidentified protein, heptoglobin, albumin, a2 globulin and unidentified protein. Their molecular weights were 150, 140, 120, 100, 60, 48 and 33 K Da and mobilities were 0.094, 0.126, 0.186, 0.268, 0.536, 0.631 and 0.805 respectively. In the FMD affected sample, g –globulin was undetectable but an extra band was noticed in the infected serum sample which was identified as the FMD viral protein.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-09-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/23654
10.56093/ijans.v82i9.23654
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 9 (2012); 1004–1005
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/23654/11338
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0