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Yak introgression into Tibetan Yellow cattle: evidences from mitochondrial DNA D-loop sequence analysis and β-lactoglobulin genetic variants

Indian Agricultural Research Journals

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Title Yak introgression into Tibetan Yellow cattle: evidences from mitochondrial DNA D-loop sequence analysis and β-lactoglobulin genetic variants
 
Creator Liu, Zhengxin
Lu, Bangmin
Mao, Yongjiang
Li, Yuping
Jin, Suyu
Zheng, Yucai
 
Subject ß-lactoglobulin
Gene introgression
mtDNA D-loop
Tibetan Yellow cattle
Yak
 
Description The objective of this study was to investigate the possible introgression of highland yak (Bos grunniens) into Tibetan Yellow cattle (Bos taurus) based on milk protein and mtDNA D-loop analysis. Tibetan Yellow cattle (43) were used to assay polymorphisms of β-lactoglobulin (β-Lg) and to extract genomic DNA for the PCR amplification of mtDNA D- loop. Native polyacrylamide gel electrophoresis (PAGE) of milk β-Lg showed that 3 milk samples exhibited the yak- specific β-Lg E variant among the 43 milk samples tested. mtDNA D-loop sequences were successfully amplified by PCR using genomic DNA extracted from milk. A total of 16 sequences of the mtDNA D-loop were obtained for analysis, which contained 133 variable sites (97 parsimony-informative sites and 36 single sites) and defined 15 different haplotypes. The 3 Tibetan Yellow cattle carrying β-Lg E variant were exactly clustered with yak when mtDNA D-loop sequences were employed for cluster analysis, while other yaks assayed (13) clustered with cattle. This research demonstrated that there existed matrilineal introgression of yak during the evolution or natural breeding of Tibetan Yellow cattle population on Qinghai-Tibet plateau.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-09-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/23657
10.56093/ijans.v82i9.23657
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 9 (2012); 1012–1015
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/23657/11341
 
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