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Rapid genomic DNA extraction for Soybean (Glycine max L. Merr) using modified CTAB protocol to obtain high-quality DNA

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Title Rapid genomic DNA extraction for Soybean (Glycine max L. Merr) using modified CTAB protocol to obtain high-quality DNA
 
Creator Irsyadi, Muhammad Burhanuddin
Sari, Supriyatin Kartika
Oktiastuti, Erlinda
Rineksane, Innaka Ageng
 
Subject CTAB
Genomic
Isolation
Purity
Quality
 
Description 97-104
Molecular and genetic analysis is one of the critical primary stages, starting with DNA extraction. DNA extraction is an
essential preliminary step in molecular research. Extracting plant genomic DNA with CTAB buffer takes approximately
three days to obtain high-quality DNA. There have yet to be reports regarding effective and efficient soybean genomic
DNA extraction methods. This study aimed to obtain a rapid extraction method for soybean with high-quality DNA. This
study used a modification of the CTAB method by shortening the duration its stages. The treatments tested were
washing soybean leaf and callus organs with ethanol one and two times. The results showed that one-time washing using
ethanol absolute obtained the highest average DNA concentrations in leaf and callus organs 458.72 ± 155 ng/μL and
363.87 ± 205 ng/μL, respectively. In addition, DNA purity was obtained 1.99 ± 0.16 and 1.86 ± 0.07, respectively, on
A260/280 ratio. Good DNA quality was indicated by the absence of smears on the 1.5% electrophoresis gel. This study
demonstrated that extracting plant DNA completed in 70 min to obtain the highest quality DNA.
 
Date 2024-01-30T11:42:59Z
2024-01-30T11:42:59Z
2024-01
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/63267
https://doi.org/10.56042/ijbb.v61i2.7603
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.61(02) [February 2024]