Record Details

Batch isolation of novel sequences targeting regions of rapid viral variations

Indian Agricultural Research Journals

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Field Value
 
Title Batch isolation of novel sequences targeting regions of rapid viral variations
 
Creator Yu, Wenwen
Yang, Xinmin
Gao, Ye
Zhang, Xiaoxia O
He, Ran
Ma, Xiaoqian
LV, Miao
Cui, Rongfeng
Feng, Shulil
Kao, Yongchang
Xie, Shaoping
Shi, Wei
Wang, Zaixue
Cui, Liang
Cao, Yan
Zhang, Ying
Wang, Yuan
Feng, Fan
Cao, Weiguo
Liu, Qiuyun
 
Subject Batch discovery
Foot-and-mouth disease virus
G-H loop
Novel sequences
Viral variations
 
Description Rapid evolution is widespread in the viral kingdom and a major concern for developing universal vaccines. The isolation of large numbers of viral sequence variants at highly variable regions in viral proteins remains a daunting challenge. Foot-and-mouth disease virus (FMDV) is a picornavirus and an underlying cause of a highly contagious disease of cloven-hoofed animals which often results in substantial economic losses. This study aimed at developing a combined method for the isolation of novel sequences to cope with rapid viral variations at the G-H loop of FMDV VP1 protein. DNA primer sets harbouring random nucleotides scattered in the G-H loop region, carrying additionally coding sequences for a T cell epitope and the carboxyl terminus, were designed and assembled by asymmetric PCR. After eliminating insert-free vector background and enriching positive clones, 100 novel sequences targeting the rapid viral variations of the G-H loop were obtained from 2 plasmid libraries. The method allowed radical changes in amino acid sequence, and our study has identified critical steps in the batch discovery of viral sequence variants, paving the way for future research on the possibility of developing a polyvalent universal vaccine.
 
Publisher Indian Council of Agricultural Research
 
Date 2012-07-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/21743
10.56093/ijans.v82i7.21743
 
Source The Indian Journal of Animal Sciences; Vol. 82 No. 7 (2012); 665–670
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/21743/10855
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0