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Development of PCR-based DNA markers for identification of hybrids of Tor putitora and Tor khudree

Indian Agricultural Research Journals

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Title Development of PCR-based DNA markers for identification of hybrids of Tor putitora and Tor khudree
 
Creator Priyadarshini, Rutuparnna
Kumar, Annam Pavan
Vivek Viswas Rao
Reang, Dhalongsaih
Pathakota, Gireesh Babu
Aparna Chaudhari
 
Subject Internal Transcribed Spacer Sequence
18S rRNA
Mahseer
DNA Markers
 
Description The genetic identification and characterization of Tor putitota, Tor khudree and their hybrids hold profoundsignificance in both fundamental biological exploration and practical domains like conservation biology andfisheries management. This study endeavors to establish a panel of PCR-based DNA markers capable ofaccurately discerning between T. putitora, T. khudree, and their hybrid counterparts. Controlled conditionswere employed to generate hybrids of T. putitora and T. khudree, serving to validate the developed markers.For hybrid identification, primers were devised targeting the nuclear DNA region of ITS1-5.8S-ITS2-28S rRNA.Post-optimization, the primers MHITSF and MH28SR yielded 700 bp and 500 bp amplicons for pure Torkhudree and Tor putitora, respectively. Remarkably, Tor putitora X Tor khudree hybrids displayed both 700 bpand 500 bp amplicons. These markers present a valuable tool for screening mahseer hybrids, therebyenhancing their management and conservation prospects.
 
Publisher Indian Fisheries Association
 
Date 2023-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/JIFA/article/view/141190
 
Source Journal of Indian Fisheries Association; Vol. 50 No. 2 (2023): Journal of Indian Fisheries Association
0972-3854
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/JIFA/article/view/141190/53923
 
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