Record Details

Development, Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (Curcuma longa L.)

DSpice at Indian Institute of Spices Research

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Creator SIJU, S
DHANYA, K
SYAMKUMAR, S
SASIKUMAR, B
SHEEJA, T E
BHAT, A I
PARTHASARATHY, V A
 
Date 2010-11-08T09:27:20Z
2010-11-08T09:27:20Z
2010
 
Identifier Molecular Biotechnology Vol.44:140–147(2010)
http://hdl.handle.net/123456789/622
 
Description Expressed sequence tags (ESTs) from turmeric
(Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I
SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed
and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
 
Format 240697 bytes
application/pdf
 
Language en
 
Subject Cross species amplification
Curcuma longa L.
Expressed sequence tags
Microsatellite markers
Turmeric
 
Title Development, Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (Curcuma longa L.)
 
Type Article