Record Details

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/12463/
https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1157678/full
https://doi.org/10.3389/fpls.2023.1157678
 
Title Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
 
Creator Kumar, M
Prusty, M R
Pandey, M K
Singh, P K
Bohra, A
Guo, B
Varshney, R K
 
Subject Abiotic Stress
Genetics and Genomics
 
Description Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches and transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery of engineered nucleases as genetic scissors to carry out precise manipulation in crop stress-responsive genes and associated molecular network has paved the way for sustainable management of abiotic stress conditions. In this context, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool has revolutionized due to its simplicity, accessibility, adaptability, flexibility, and wide applicability. This system has great potential to build up crop varieties with enhanced tolerance against abiotic stresses. In this review, we summarize the latest findings on understanding the mechanism of abiotic stress response in plants and the application of CRISPR/Cas-mediated gene-editing system towards enhanced tolerance to a multitude of stresses including drought, salinity, cold, heat, and heavy metals. We provide mechanistic insights on the CRISPR/Cas9-based genome editing technology. We also discuss applications of evolving genome editing techniques such as prime editing and base editing, mutant library production, transgene free and multiplexing to rapidly deliver modern crop cultivars adapted to abiotic stress conditions.
 
Publisher Frontiers Media
 
Date 2023-04-18
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_attribution
 
Identifier http://oar.icrisat.org/12463/1/Frontiers%20in%20Plant%20Science_14_01-22_2023.pdf
Kumar, M and Prusty, M R and Pandey, M K and Singh, P K and Bohra, A and Guo, B and Varshney, R K (2023) Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants. Frontiers in Plant Science (TSI), 14. 01-22. ISSN 1664-462X