Record Details

Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies

DSpice at Indian Institute of Spices Research

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Creator LINCY, A K
SASIKUMAR, B
 
Date 2010-11-08T09:28:14Z
2010-11-08T09:28:14Z
2010
 
Identifier Turkish Journal of Botany Vol.34: 21-29 (2010)
http://hdl.handle.net/123456789/625
 
Description A protocol for direct plantlet regeneration from aerial stem explants of ginger (Zingiber officinale Rosc.) has been developed. Adventitious shoots and roots were regenerated from in planta aerial stem explants of 2 ginger varieties viz. var. ‘Jamaica’ and var. ‘Varada’. Maximum shoot and root regeneration were observed in the cultures containing TDZ and IBA (1:1; 1:0.1 mg L-1) in both varieties. Cultures containing TDZ alone showed poor shoot and root formation.Histological studies revealed that the shoot initials originated from the primary thickening meristem and apical meristem,while the root primordial originated from the primary thickening meristem present in the aerial stem. The hardening of in vitro plants was performed with different hardening media and successful acclimatization was obtained. The hardening
medium containing soil: sand: coir dust: cow dung + 5 g Trichoderma harzianum/ cup gave good result in all aspects like survival of hardened plants, plant height, number of leaves, and chlorophyll content.
 
Format 446431 bytes
application/pdf
 
Language en
 
Subject Aerial stem
Biological hardening
Direct regeneration
Ginger
Trichoderma harzianum
 
Title Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies
 
Type Article