ICAR Research Data Repository for Knowledge Management
Standardization of DNA isolation and PCR parameters in Garcinia spp. for RAPD analysis
DSpice at Indian Institute of Spices Research
View Archive Info| Field | Value | |
| Creator |
ASISH, G R
UTPALA PARTHASARATHY NITHYA, N G |
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| Date |
2011-01-22T05:15:40Z
2011-01-22T05:15:40Z 2010 |
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| Identifier |
Indian Journal of Biotechnology Vol.9(4) 2010
0975-0967 http://hdl.handle.net/123456789/637 |
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| Description |
Doyle and Doyle protocol with a little modification was used for the isolation of DNA from leaves and fruit rind of Garcinia spp. In case of leaves and dry fruit rind, the best DNA isolation was possible with 4% CTAB (100 mM Tris, 30 mM EDTA & 1.4 M NaCl), followed by 1.5% PVP and 0.3% mercaptoethanol. In case of fresh fruit rind, the DNA was best isolated with 2% CTAB (100 mM Tris, 30 mM EDTA & 1.4 M NaCl). PCR parameters were optimized using random RAPD primers. Other parameters included the DNA concentration at 50 ng/reaction, annealing temperature of 43°C, 2.5 mM dNTP in presence of 1 U Taq DNA polymerase and 2.0 mM MgCl2. The protocol will be of great help to study the genetic diversity of Garcinia germplasms.
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| Format |
388192 bytes
application/pdf |
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| Language |
en
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| Publisher |
NISCAIR-CSIR, India
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| Subject |
DNA extraction
Garcinia fresh fruit rind dry fruit rind RAPD-PCR |
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| Title |
Standardization of DNA isolation and PCR parameters in Garcinia spp. for RAPD analysis
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| Type |
Article
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