Record Details

Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture

DSpice at Indian Institute of Spices Research

View Archive Info
 
 
Field Value
 
Creator RETHEESH, S T
BHAT, A I
 
Date 2011-01-24T10:26:10Z
2011-01-24T10:26:10Z
2010-05
 
Identifier Crop Protection 29 :1214 -1217 (2010)
0261-2194
http://hdl.handle.net/123456789/648
 
Description Vanilla (Vanilla planifolia G. Jackson), a perennial fleshy vine infected by Cucumber mosaic virus (CMV)and Cymbidium mosaic virus (CymMV) was freed from infection through meristem culture. Apical meristem measuring 0.1e0.25 mm were isolated and cultured in Murashige and Skoog (MS) medium supplemented with 0.45 mM thidiazuron for 40e45 days to initiate the growth. Following enlargement of
meristem, it was transferred to MS medium supplemented with 4.43 mM 6-benzyl aminopurine (BAP)and 2.68 mM a-naphthalene acetic acid (NAA) for regeneration. The regenerated plantlets were hardened in insect-free glasshouse and confirmed for the elimination of viruses using reverse transcription PCR (RT-PCR) with virus specific primer pairs. The frequency of CMV elimination was 79.4% while that of CymMV was 82.4% when tested individually. We obtained a frequency of 75% simultaneous elimination of both viruses. This is the first report of meristem culture and virus elimination in vanilla.
 
Format 1654126 bytes
application/pdf
 
Language en
 
Publisher Elsevier
 
Subject Cucumber mosaic virus
Cymbidium mosaic virus
Meristem culture
RT-PCR
Vanilla
Virus elimination
 
Title Simultaneous elimination of Cucumber mosaic virus and Cymbidium mosaic virus infecting Vanilla planifolia through meristem culture
 
Type Article