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Optimization studies of L-glutaminase production from Halomonas hydrothermalis B-15-9-2 isolated along Gujarat coasts

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Title Optimization studies of L-glutaminase production from Halomonas hydrothermalis B-15-9-2 isolated along Gujarat coasts
 
Creator Patel, Nidhi Y
Parmar, Fenil
Patel, Jay
Dighe, Harshali
Raval, Shivani Yagnik
Raval, Vikram H
 
Subject Halophiles
Marine enzymes
Molecular identification
Physicochemical analysis
Response surface methodology
 
Description 153-165
L-glutaminase (EC 3.5.1.2) is an enzyme grouped under amidohydrolase and a member of serine-dependent β-lactamases
& penicillin-binding proteins. The present study focuses on increasing the yield of L-glutaminase from marine bacteria
isolated from coastal regions of Gujarat. Using enrichment culture technique marine samples were enriched and
physicochemical analysis was done. A total of 12 bacteria isolated from Gujarat coasts were screened for L-glutaminase
biosynthesis. Out of 12, most potent one isolate B-15-9-2 was selected for cultural, biochemical, and molecular
identification by 16S rRNA analysis, which reveals the nearest homolog is Halomonas hydrothermalis with 99.87%
similarity. To the best of our knowledge this is the first report of Halomonas hydrothermalis as L-glutaminase producer
from Gujarat coasts. Further study deals with the optimization of medium components for higher yield of L-glutaminase by
one variable at a time & response surface methodology. There was a significant increase in the L-glutaminase activity about
3.4-fold from 29 ± 0.8 U/mL to 98.95 ± 0.5 U/mL upon media optimization via statistical method.
 
Date 2024-02-21T06:56:14Z
2024-02-21T06:56:14Z
2024-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/63366
https://doi.org/10.56042/ijbb.v61i3.7621
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.61(03) [March 2024]