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Investigation of antioxidant and antibacterial activities, phenolic contents of Allium staticiforme bulb fractions in different polarities

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Title Investigation of antioxidant and antibacterial activities, phenolic contents of Allium staticiforme bulb fractions in different polarities
 
Creator Semerci, Alican Bahadır
Tunç, Kenan
Sağiroğlu, Mehmet
 
Subject Allium staticiforme
Antibacterial activity
Antioxidant activity
Solvent
 
Description 182-188
In this study, it was aimed to evaluate the antioxidant and antimicrobial activities of Allium staticiforme bulb fractions
(petroleum ether, chloroform, methanol and acetone) in vitro. Fractions were made by Soxhlet method, antibacterial activity was
evaluated using disc diffusion method, antioxidant activity was evaluated using DPPH radical scavenging detection and reduction
power test. At the same time, the chemical content was determined by examining TLC and total phenolic matter. The
concentrations reducing the DPPH radical of the fractions by 50% were determined to be 283.1 μg/mL for methanol, 291.5 μg/mL
for petroleum ether, 346 μg/mL for chloroform and 533.6 μg/mL for acetone, respectively. It was observed that the total phenolic
substance amounts were in the order (from the higher to the lower): petroleum ether, methanol, chloroform and acetone fractions.
The reduction power was directly proportional to the increase in the concentration for all fractions. The highest antibacterial effect
was found on the petroleum ether fraction (12 mm) on S. aureus. It was determined that the petroleum ether fraction showed a
broad spectrum of antibacterial activity. In the current study the antibacterial and the antioxidant activity of Allium staticiforme bulb
fractions (with different polarity) have been investigated for the first time and shown that the solution used in extract preparation is
important in revealing the bioactive molecules.
 
Date 2024-02-22T11:05:00Z
2024-02-22T11:05:00Z
2024-02
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/63381
https://doi.org/10.56042/ijtk.v23i2.8725
 
Language en
 
Relation Int Cl.24: A61K 36/00
 
Publisher NIScPR-CSIR,India
 
Source IJTK Vol.23(2) [February 2024]