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Pathological analysis and antimicrobial susceptibility of Chryseobacterium balustinum RTFCP 298 isolated from diseased rainbow trout, Oncorhynchus mykiss

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Title Pathological analysis and antimicrobial susceptibility of Chryseobacterium balustinum RTFCP 298 isolated from diseased rainbow trout, Oncorhynchus mykiss
 
Creator Sumanta Kumar Mallik
Neetu Shahi
Ritesh Shantilal Tandel
Debajit Sarma, Pramod Kumar Pandey
 
Subject rainbow trout, Oncorhynchus mykiss
Chryseobacterium balustinum
 
Description Not Available
In this study, six isolates of Chryseobacterium balustinum were characterized from diseased rainbow
trout fngerlings. The virulence characteristics, pathogenicity, and antimicrobial susceptibility
pattern of these isolates were investigated. The bacterium showed positive results for catalase,
cytochrome oxidase, and aesculin hydrolysis, while negative results were obtained for DNase,
gelatinase, methyl red, Voges-Proskauer’s reaction, Simon citrate, Hydrogen sulphide, and starch
hydrolysis. Amino acid metabolism analysis revealed the inability to metabolize arginine, lysine, and
ornithine decarboxylase. Molecular characterization (16S rRNA) and phylogenetic analysis revealed
the test isolates as C. balustinum, closely related to strain WLT (99.85% similarity) and C. balustinum
P-27 (99.77%). Virulence assay indicated haemolytic activity and bioflm formation by the test
bacterium. The challenge test confrmed moderate pathogenicity in rainbow trout and established
Koch’s postulates. The clinical manifestations of infection included fn erosion, eye and body surface
haemorrhage, exophthalmia, and organ liquefaction. The minimum inhibitory concentrations of
various antimicrobials ranged from 1 to> 256 µg ­mL−1. The novel synthetic antimicrobial peptides
exhibited MICs of 8 to> 256 µg ­mL−1, suggesting a potential control method. These fndings
suggest that C. balustinum is an opportunistic pathogen with moderate pathogenicity in rainbow
trout. Further research on the host–pathogen relationship is necessary to understand virulence
characteristics and pathogenicity in aquaculture.
Not Available
 
Date 2024-02-28T20:22:40Z
2024-02-28T20:22:40Z
2023-08-15
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/81526
 
Language English
 
Relation Not Available;
 
Publisher Not Available