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Biodegradation of Low Density Polyethylene (LDPE) using marine bacteria isolated from tropical beaches of megacity Mumbai

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Title Biodegradation of Low Density Polyethylene (LDPE) using marine bacteria isolated from tropical beaches of megacity Mumbai
 
Creator Fulke, A B
Khade, K
Sasidharan, A K
 
Subject Bacillus
Lysinibacillus
Marine microbes
Plastic degradation
Pseudomonas
 
Description 182-188
Plastics, although as useful and versatile as they are, have led to major environmental pollution. Its resistance to degradation has
caused a major global threat to the environment. Plastic waste is disposed of in landfills causing soil pollution, incinerated releasing
toxic fumes and causing air pollution, and dumped in the ocean causing a wide range of problems like entanglement and ingestion
by marine creatures. Since the introduction of plastic, microorganism, though incapable of degrading it, is known to associate with
its surface. Marine ecosystem is one such environment where the disposal of a large amount of plastic waste is found, and
microorganisms are known to be associated with it. Hence, the marine environment can serve as a potential source for indigenous
plastic-degrading microorganisms. In the following study, plastic pieces rooted deep in sediments from seven recreational beaches
in Mumbai were collected for the isolation of possible plastic degrading bacteria associated with the surface of the collected plastic
pieces. Altogether, nine isolates were isolated after three months of incubation of collected samples in a medium containing no
carbon source. On identification by 16S rRNA sequencing, isolates were confirmed to be Pseudomonas, Bacillus and
Lysinibacillus. The NCBI accession numbers were obtained for all the isolates. For further experimentation, only four
Pseudomonas isolates (Strains ABFPD01, ABFPD02, ABFPD03 and ABFPD05) and one each isolate of Bacillus and
Lysinibacillus were used. In the weight loss experiment, the ABFPD02 & ABFPD03 strains of Pseudomonas degraded 11 % of
pre-weighed polyethylene strips in 2 months incubation period. The marine environment needs to be studied extensively for more
potential microorganisms for plastic degradation.
 
Date 2024-03-01T05:11:44Z
2024-03-01T05:11:44Z
2024-02
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/63503
https://doi.org/10.56042/ijms.v52i04.7648
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJMS Vol.52(04) [April 2023]