Record Details

Occurrence of Banana bract mosaic virus on cardamom

DSpice at Indian Institute of Spices Research

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Field Value
 
Creator SILJO, A
BHAT, A I
BIJU, C N
VENUGOPAL, M N
 
Date 2011-11-12T18:23:42Z
2011-11-12T18:23:42Z
2012
 
Identifier Phytoparasitica 40:77–85 (2012)
1876-7184
http://hdl.handle.net/123456789/713
 
Description During a survey for mosaic disease caused
by Cardamom mosaic virus (CdMV) in cardamom
plantations, a new kind of viral disease showing chlorotic streak on veins was observed in some plantations. Based on the type of symptom, the disease was named ‘chlorotic streak’. In a survey of 77 cardamom plantations at 49 locations in the states of Kerala, Karnataka and Tamil Nadu in India, incidence of the disease ranged from zero to 15%. The plants infected with chlorotic streak tested negative in reverse transcription polymerase chain reaction (RT-PCR) for CdMV. Leaf dip electron
microscopy of these samples showed the presence of flexuous virions resembling Potyvirus. Total RNA extracted from these plants when subjected to RT-PCR using primers targeted to conserved region in Potyvirus resulted in an amplicon size of ~700 bp. Sequencing and BLAST analysis of this amplicon showed Banana bract mosaic virus (BBrMV) as the closest virus. Using primers specific for BBrMV, the coat protein gene from five different geographical
isolates of the virus was cloned and sequenced. Sequence analysis of the coat protein gene showed an identity of >94% with BBrMV isolates while identity with other distinct potyvirus species was
 
Format 884075 bytes
application/pdf
 
Language en
 
Publisher Springer
 
Subject BBrMV
Cardamom mosaic virus
Chlorotic streak
Coat protein
Detection
Elettaria cardamomum
RT-PCR
 
Title Occurrence of Banana bract mosaic virus on cardamom
 
Type Article